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Cloning and Expression
Enzymes
Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL
Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL from Arraystar, Inc
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Citations:
(19)
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Arraystar, Inc for
Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL
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Description
Arraystar SYBR® Green qPCR Master Mix contains all the components for an optimized qPCR reaction, with high stability, excellent repeatability and high specificity
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL
Company
Arraystar, Inc
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
AS-MR-006-5
Quantity
5mL
Type
Master Mix
Research Area
PCR
Applications
qPCR
Specifications/Features
All components included, just mix with cDNA template; Convenient 2X concentrate; Hot start polymerase for high specificity; Stabilized for consistent high performance; ROX passive reference included.
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Arraystar, Inc
9430 Key West Avenue
Suite 128
Rockville
,
Maryland
20850
United States
Phone
: 888-416-6343
240-314-0388
Fax: 240-238-9860
Company Profile
Website:
http://www.arraystar.com
Citations (19)
(1)
N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing
Nucleic Acids Research
August 11, 2023
Can Wang, Nathalie Ulryck, Lydia Herzel, Nicolas Pythoud, Nicole Kleiber, Vincent Guérineau, Vincent Jactel, Chloé Moritz, Markus T Bohnsack, Christine Carapito,
et al.
nrStar Human tRNA PCR Array
(
Arraystar
, #
AS-NR-001–1
) and Arraystar SYBR Green qPCR Master Mix(ROX+) (
Arraystar
; #
AS-MR-006–5
). The data were analyzed by the ΔCt method. The ΔCt values were calculated for each tRNA using the following
(2)
Analysis of mRNA m6A modification and mRNA expression profiles in middle ear cholesteatoma
Front Genet
August 7, 2023
Shumin Xie, Li Jin, Jun He, Jinfeng Fu, Tuanfang Yin, Jihao Ren, Wei Liu
generate cDNA, and the Arraystar SYBR® Green qPCR Master Mix (
Arraystar
, Rockville, MD, USA, Cat. No.
ASMR-006-5
) was used for quantification. Three replicates were tested for each sample. Data were normalized to
(3)
CircRNA expression profiles and functional analysis in a mouse model of chronic intermittent hypoxia-induced renal injury: new insight into pathogenesis
PeerJ
February 28, 2023
Qingshi Chen, Zhenzhen Hong, Zhiyu Chen, Yanfeng Chen, Dexin Liu
(18080-044;
Invitrogen
, Waltham, MA, USA). According to the manufacturer’s instructions, 2X PCR Master Mix (
AS-MR-006-5
;
Arraystar
, Rockville, MD, USA) and
ViiA 7 Real-Time PCR system
(
Applied Biosystems
, Waltham, MA, USA)
(4)
Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semen
Molecular Human Reproduction
February 28, 2023
Germán Gornalusse, Ryan M Spengler, Erin Sandford, Yeseul Kim, Claire Levy, Muneesh Tewari, Florian Hladik, Lucia Vojtech
from each donor (in a
1:40
dilution) were mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (
AS-MR-006-5
,
Arraystar
, Rockville, MD, USA) and loaded onto the 384-well
PCR
array. As controls, we included: genomic
(5)
Differential expression profiling of tRNA-Derived small RNAs and their potential roles in methamphetamine self-administered rats
Frontiers in Genetics
February 2, 2023
Yun Zhou, Qingxiao Hong, Wenjin Xu, Weisheng Chen, Xiaohu Xie, Dingding Zhuang, Miaojun Lai, Dan Fu, Zemin Xu, Majie Wang,
et al.
RNA-seq
were included. The amplified cDNA was mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (
AS-MR-006-5
,
Arraystar
). Serially diluted DNA templates were prepared for standard curve drawing. All cDNA samples
(6)
Differentially expressed tRNA-derived fragments and their roles in primary cardiomyocytes stimulated by high glucose
Front Endocrinol (Lausanne)
January 13, 2023
Yongting Zhao, Ruxin Wang, Qi Qin, Jiaojiao Yu, Hui Che, Lihong Wang
hybridization
, 5’ adaptor
ligation
and reverse transcription. Arraystar SYBR® Green qPCR Master Mix (ROX+) (
AS-MR-006-5
,
Arraystar
, USA) was used to perform
qRT-PCR
, amplifying and quantifying the cDNA according to the manufacturer’s
(7)
Circular RNAs expression profiles and bioinformatics analysis in bronchopulmonary dysplasia
Journal of Clinical Laboratory Analysis
January 1, 2023
Yu Lun, Junlong Hu, Yang Zuming
20.0 μl with RNase‐free water.
QRT‐PCR
analyses were carried out using 2 × PCR Master Mix (
Arraystar
:
AS‐MR‐006‐5
). NCBI Primer‐BLAST and Generay Biotech were utilized to design and manufacture all primers. The 20
(8)
The m6A methylation profiles of immune cells in type 1 diabetes mellitus
Frontiers in Immunology
November 15, 2022
Yimeng Wang, Linling Xu, Shuoming Luo, Xiaoxiao Sun, Jiaqi Li, Haipeng Pang, Jun Zhou, Yuemin Zhou, Xiajie Shi, Xia Li,
et al.
Cat.No.
RR037A
). We prepared the Arraystar SYBR® Green qPCR Master Mix (
Arraystar
, Rockville, MD, USA, Cat.No.
AS-MR-006-5
) in each tube and added the RNA samples to the tubes containing Master Mix on ice. The tubes were incubated
(9)
RNA â€sequencing reveals the expression profiles of tsRNAs and their potential carcinogenic role in cholangiocarcinoma
Journal of Clinical Laboratory Analysis
October 1, 2022
Yanâ€ke Li, Liâ€rong Yan, Ang Wang, Liâ€yue Jiang, Qian Xu, Benâ€gang Wang
control.
Quantitative Real‐time PCR
was performed by
Arraystar
SYBR Green Real‐Time
qPCR Master Mix
(Cat#
AS‐MR‐006‐5
). The relative expression level of each tRNA‐derived fragments was calculated with 2−ΔCt. SPSSv24.0
(10)
Identification of Small Nucleolar RNA SNORD60 as a Potential Biomarker and Its Clinical Significance in Lung Adenocarcinoma
BioMed Research International
June 7, 2022
Hongwei Zhou, Yibing Yao, Yan Li, Nannan Guo, Huanhuan Zhang, Zhikuan Wang, Yingtai Chen, Guanghai Dai
(Cat#
AS-FS-001
,
Arraystar
, US), cDNA was mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (
AS-MR-006-5
,
Arraystar
), and snoRNA profiling was performed by using
nrStar™ Human snoRNA PCR array
according to
(11)
RNA sequencing reveals novel LncRNA/mRNAs co-expression network associated with puerarin-mediated inhibition of cardiac hypertrophy in mice
PeerJ
April 5, 2022
Shan Ye, Weiyan Chen, Caiwen Ou, Min-Sheng Chen
Real-time PCR System
(Applied Biosystems, Waltham, MA, USA) with the 2 × PCR Master Mix (
Arraystar
:
AS-MR-006-5
). Airn (antisense of Igf2r non-protein coding RNA) expression was determined using the 5′-CTAAGCCACTGCCGTCCATA-3′
(12)
Differential Expression Profiles and Bioinformatics Analysis of tRNA-Derived Small RNAs in Muscle-Invasive Bladder Cancer in a Chinese Population
Genes (Basel)
March 28, 2022
Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tian
ViiA 7 Real-time PCR System
(
Applied Biosystems
, Waltham, MA, USA) using a 2× PCR master mix (
Arraystar
:
AS-MR-006-5
). The parameter settings were as follows: 95 °C denaturation (10 min), 95 °C (10 s), and 60 °C (60 s),
(13)
The Involvement of Long Non-coding RNA and Messenger RNA Based Molecular Networks and Pathways in the Subacute Phase of Traumatic Brain Injury in Adult Mice
Frontiers in Neuroinformatics
March 4, 2022
Zhaoyu Yang, Xuexuan Li, Weikang Luo, Yao Wu, Tao Tang, Yang Wang
SuperScript™ III Reverse Transcriptase
kit (
Invitrogen
, 18080-044).
qRT-PCR
was performed using 2 × PCR master mix (
Arraystar
,
AS-MR-006-5
) in a Thermal Cycler Dice Real-Time System II. The primer sequences are displayed in Table 1. All experiments
(14)
Differential Expression and Bioinformatics Analysis of tRF/tiRNA in Endometriosis Patients
BioMed Research International
March 3, 2022
Wang-shu Li, Yi-lin Li, Rui Cao, Chun-fang Ha, Shu Sun, Lan Yu, Jian Li
Quantitative PCR
Amplification
The obtained cDNA was mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (
AS-MR-006-5
,
Arraystar
) and added to a 384-well plate. The
real-time PCR
amplification
was performed on the ABI
(15)
Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats
Frontiers in neuroscience
January 1, 2022
Xing M, Deng M, Shi Y, Dai J, Ding T, Song Z, Zou W.
enzymes are shown in Supplementary Table 1.
Real-time PCR
reaction using PCR master mix (
Arraystar
,
AS-MR-006-5
) was performed according to the manufacturer’s manual in the ViiA7
Real-Time PCR
Detection System (Applied
(16)
Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells
PLoS genetics
November 1, 2021
Chen M, Long Q, Borrie MS, Sun H, Zhang C, Yang H, Shi D, Gartenberg MR, Deng W.
nrStar Human tRNA PCR Array
(
Arraystar
AS-NR-001-1
) and Arraystar SYBR Green qPCR Master Mix(ROX+) (
Arraystar
AS-MR-006-5
). The data were analyzed by the ΔΔCt method. RNA immunoprecipitation (RIP) RIP was performed following
(17)
Differential Expression Profiles and Function Predictions for tRFs & tiRNAs in Skin Injury Induced by Ultraviolet Irradiation
Frontiers in Cell and Developmental Biology
August 10, 2021
Yuan Fang et al.
First-Strand cDNA Synthesis Kit
(Cat#
AS-FS-003
,
Arraystar
), respectively. The 2× PCR master mix (
Arraystar
:
AS-MR-006-5
) was used for the
PCR
through
ViiA 7 Real-time PCR System
(
Applied Biosystems
). The reactions were incubated
(18)
Pathophysiological Significance of Neutrophilic Transfer RNA-Derived Small RNAs in Asymptomatic Moyamoya Disease
Cells
May 1, 2021
Lingzhi Li et al.
performed
qRT-PCR
in the
ViiA 7 Real-time PCR System
(
Applied Biosystems
) using a 2× PCR master mix (
AS-MR-006-5
;
Arraystar Inc
., Rockville, MD, USA). The relative Cells 2021, 10, 1086 4 of 11 tsRNA expression
(19)
Distinct Circulating Expression Profiles of Long Noncoding RNAs in Heart Failure Patients With Ischemic and Nonischemic Dilated Cardiomyopathy
Frontiers in Genetics
November 12, 2019
Fang Lin et al.
ViiA 7 Real-Time PCR System
(Thermo Fisher Scientific, Waltham MA, USA) and 2*PCR Master Mix (
Arraystar Inc
., catalog #
AS-MR-006-5
, Rockville, MD, USA). Primers were designed online using Primer 5 software and evaluated by the Basic
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Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL from Arraystar, Inc
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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